Polymerization of carbamylated deoxyhemoglobin S in concentrated phosphate buffer.
نویسندگان
چکیده
منابع مشابه
Gelation of deoxyhemoglobin A in concentrated phosphate buffer. Exhibition of delay time prior to aggregation and crystallization of deoxyhemoglobin A.
We found that deoxy Hb A aggregated or formed gels with a clear exhibition of a delay time when a solution of deoxy Hb A with a concentration of 120% of its solubility was incubated at 30°C. Since the solubility of deoxy Hb A is much higher than that of deoxy Hb S, this condition was created by increasing the phosphate concentration. The length of the delay and aggregation times and the amounts...
متن کاملAggregation of hemoglobin S and hemoglobin CHarlem with nonsickle hemoglobin in concentrated phosphate buffer.
The effect of HbA and HbF on both the aggregation and deaggregation of deoxy HbS and deoxy HbCHarlem in concentrated phosphate buffers was studied by a turbidimetric method. Although pure deoxy HbA is fully soluble in 2.4 M potassium phosphate buffer, the same concentration of deoxy HbAS was insoluble in the same buffer. Since there was little dissolved hemoglobin in the solute of a mixture of ...
متن کاملThe solubility of sickle and non-sickle hemoglobins in concentrated phosphate buffer.
A new turbidimetric method for the direct measurement of the solubility of oxy- and deoxyhemoglobins (Hb) in concentrated phosphate buffer has been established. The principle of the method is the formation of a homogeneous emulsion when hemoglobin is introduced in concentrated phosphate buffer. The solubility of the oxy and deoxy forms of Hb A, Hb S, Hb C, Hb F, and Hb CHarlem (beta 6Glu leads ...
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The reduction mechanism of molybdenum-methyl thymol blue (MTB)-chlorate system has been investigated in water on a mercury electrode. As this system is very sensitive for ultratrace determination of molybdenum, the actual mechanism involved was studied. Electrochemical techniques, including dc tast polarography and cyclic voltammetry were employed. It is shown that, the cathodic wave of mol...
متن کاملDetermination of deoxyhemoglobin S polymer in sickle erythrocytes upon deoxygenation.
We have used 13C/1H magnetic double-resonance spectroscopy to measure the amount of sickle hemoglobin polymer within sickle erythrocytes as a function of oxygen saturation. We previously showed that the methods of cross-polarization and scalar decoupling could be used to measure accurately the polymer fraction in deoxygenated sickle hemoglobin solutions [Noguchi, C.T., Torchia, D.A. & Schechter...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)33593-2